Journal
RSC ADVANCES
Volume 9, Issue 44, Pages 25318-25325Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9ra04513a
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Funding
- National Key R&D Program of China [2018YFC0910602]
- National Natural Science Foundation of China [81627807, 11727813, 81571725, 91859109, 81871397, 81701853, 81660505]
- Fok Ying-Tong Education Foundation of China [161104]
- Program for the Young Top-notch Talent of Shaanxi Province
- Research Fund for Young Star of Science and Technology in Shaanxi Province [2018KJXX-018]
- Natural Science Basic Research Plan in Shaanxi Province of China [2018JM7072, 2019JQ-519, 2019JQ-045]
- Fundamental Research Funds for the Central Universities [JB181203, JB191201]
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Molecular imaging is a powerful tool for non-invasive visualization of tumors that plays an important role in their diagnosis and treatment. The specificity of molecular imaging probes for cancer cells is important for accurate tumor visualization, with antibody and polypeptide nanoprobe conjugates having often been used as targeting agents for tumor detection. However, many traditional chemical conjugation methods employ complex conjugation reactions that result in poor efficiency and poor bioactivity. Herein, we describe the use of photo click methodology for the rapid synthesis of nanoprobes comprised of silica nanoparticles functionalized with RGD targeting units (SiO2@T1-RGDk NPs) (similar to 80 nm) for in vivo prostate cancer fluorescent imaging applications. These SiO2@T1-RGDk NPs exhibit a maximum absorption wavelength of 380 nm in their UV absorption spectra with a maximum fluorescence emission wavelength of 550 nm. Confocal immunofluorescent imaging reveal that SiO2@T1-RGDk NPs exhibit excellent targeting ability for visualizing cancer cells, with in vivo fluorescence imaging intensity in a subcutaneous tumor model of prostate cancer reaching a maxima after 4 h. Biosafety assessments showed that SiO2@T1-RGDk NPs demonstrate no obvious toxicity in mice, thus demonstrated that these novel NPs may prove to be promising fluorescent imaging agents for the accurate detection and treatment of tumors.
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