Journal
PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY
Volume 88, Issue 2, Pages 139-147Publisher
TECH SCIENCE PRESS
DOI: 10.32604/phyton.2019.06546
Keywords
Annonaceae; antioxidant activity; 6-benzyladenine; secondary metabolites; tissue culture
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Callus cultures of Annona muricata and Annona purpurea were induced in Murashige and Skoog (MS) medium supplemented with different concentrations of 1-naphthylacetic acid (NAA), 6-benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D) utilized hypocotyls with explant. The highest percentage of callus formation was the treatment supplemented with 3 mg L-1 NAA for A. muricata (100%) while for A. purpurea in lower percentage (75%). BA stimulated the formation of shoots in all the evaluated concentrations, being the concentration of 2 mg L-1 the one that induced the greater formation of shoots for A. muricata (23 shoots/explant) and A. purpurea (28 shoots/explant). The content of total phenols, flavonoids and antioxidant activity was measured in the callus obtained from both species. The results showed that a higher content of total phenols was quantified in callus of A. purpurea (27.8 mg g(-1) dw) compared to A. muricata (23.2 mg g(-1) dw). The highest content of total flavonoids was observed in the callus of A. purpurea (8.0 ng g(-1) dw). Antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydracil radical assay. The concentration required for 50% inhibition (IC50) of the 2,2-diphenyl-1-picrylhydracil radicals were 4.22 mu g mL(-1) in methanolic extracts of callus of A. muricata, while in extracts of callus of A. purpurea was 2.86 mu g mL(-1), in both cases was greater than that found for leaves. Callus culture of the species studied in this work represents an alternative for the production of natural antioxidants.
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