Journal
JOURNAL OF SEPARATION SCIENCE
Volume 40, Issue 9, Pages 1993-2001Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201700045
Keywords
derivatization; 4-nitrobenzoyl chloride; high-performance liquid chromatography; semicarbazides
Categories
Funding
- National Natural Science Foundation of China [21475153, 21475154, 21675178]
- Guangdong Provincial Natural Science Foundation of China [2015A030311020]
- Special Funds for Public Welfare Research and Capacity Building in Guangdong Province of China [2015A030401036]
- Guangzhou Science and Technology Program of China [201604020165]
Ask authors/readers for more resources
Semicarbazide, a toxic food contaminant, widely exists in food products and it originates from the thermal degradation of a food additive of azodicarbonamide or a metabolite of nitrofurazone abused in meat specimens. Many previous methods for semicarbazide determination usually required expensive instruments, difficult-to-prepare monoclonal antibodies, and a long operation time. In this study, a high-performance liquid chromatography method was developed for the rapid determination of trace semicarbazide coupling with a nucleophilic substitution reaction firstly using 4-nitrobenzoyl chloride as derivatization reagent. The derivatization reaction was mild at room temperature for 1 min in neutral solution. Then, semicarbazide derivative was separated and quantified by high-performance liquid chromatography with ultraviolet detection under optimal separation conditions at lambda(max) = 261 nm. The proposed method offered the detection limit of 1.8 mu g/L and was successfully applied for the rapid determination of trace semicarbazide in flour products. Semicarbazide in positive real samples could be actually found and quantified in the range of 0.47-7.53 mg/kg. The recoveries were 76.6-119% with relative standard deviations of 0.5-9.1% (n = 3). This developed method was rapid, reliable, and convenient for the determination of trace semicarbazide in food.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available