Journal
JOURNAL OF SEPARATION SCIENCE
Volume 40, Issue 9, Pages 1877-1886Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201601438
Keywords
inhibitors; magnetic beads; natural products; xanthine oxidase
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Funding
- National Natural Scientific Foundation of China [81473317, 81473555]
- Qing Lan Project
- Science and Technology Development Fund of Macao SAR [013/2015/A1]
- Program for Excellent Scientific and Technological Innovation Team of Jiangsu Higher Education
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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In this study, high-performance liquid chromatography coupled with tandem mass spectrometrywas used to assess the results of bioactive compound screening from natural products using immobilized enzyme magnetic beads. We compared three commercial magnetic beads with modified amino, carboxy, and N-hydroxysuccinimide groups, respectively. Amino magnetic beads performed best for immobilization and were selected for further experiments. Xanthine oxidase was immobilized on amino magnetic beads and applied to screen potential inhibitors in fresh Zingiber officinale Roscoe, extracts of Scutellaria baicalensis Georgi, and Pueraria lobata Ohwi. In total, 12 potential xanthine oxidase ligands were identified from fresh Zingiber root and Scutellaria root extracts, of which eight were characterized and the concentration required for 50% inhibition was determined. Preliminary structure-function relationships were discussed based on these results. A convenient and effective method was therefore developed for the identification of active compounds from complex natural product mixtures.
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