Journal
DISEASE MODELS & MECHANISMS
Volume 8, Issue 3, Pages 311-321Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dmm.018366
Keywords
Fibrosis; Larynx; Scar formation; Tissue repair; Transcriptome
Categories
Funding
- National Institute on Deafness and other Communication Disorders [R01 DC004428, R01 DC010777]
- Clinical and Translational Science Award (CTSA) program of the National Center for Research Resources [UL1 RR025011]
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The vocal fold (VF) mucosa confers elegant biomechanical function for voice production but is susceptible to scar formation following injury. Current understanding of VF wound healing is hindered by a paucity of data and is therefore often generalized from research conducted in skin and other mucosal systems. Here, using a previously validated rat injury model, expression microarray technology and an empirical Bayes analysis approach, we generated a VF-specific transcriptome dataset to better capture the system-level complexity of wound healing in this specialized tissue. We measured differential gene expression at 3, 14 and 60 days post-injury compared to experimentally naive controls, pursued functional enrichment analyses to refine and add greater biological definition to the previously proposed temporal phases of VF wound healing, and validated the expression and localization of a subset of previously unidentified repair-and regeneration-related genes at the protein level. Our microarray dataset is a resource for the wider research community and has the potential to stimulate new hypotheses and avenues of investigation, improve biological and mechanistic insight, and accelerate the identification of novel therapeutic targets.
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