Journal
PHYSICAL REVIEW E
Volume 100, Issue 3, Pages -Publisher
AMER PHYSICAL SOC
DOI: 10.1103/PhysRevE.100.032404
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Funding
- DST, Govt. of India
- Directorate of Minorities, Govt. of Karnataka, India
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We have studied the kinetics of the interaction between antimicrobial peptide nisin and Langmuir monolayers of phospholipids DPPC and DPPG at the air-water interface using the surface manometry technique. The charge on the nisin and the lipid molecules is controlled by varying the pH of the subphase, and the interactions between them are studied by measuring the surface pressure of the lipid monolayer as a function of time after injecting the nisin in the subphase. A model based on the diffusion of particles under the influence of a constant force is developed to obtain an analytical expression for surface pressure as a function of time. The expression was found to fit well with the experimental data. The average hydrodynamic radius and the translational diffusion constant of the nisin molecules are calculated from the fit parameters for the different subphase pH solutions.
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