Journal
JOURNAL OF PROTEOME RESEARCH
Volume 16, Issue 4, Pages 1763-1772Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.7b00024
Keywords
pancreatic cancer; exosomes; iTRAQ; chemotherapy; chemoradiation; metastasis
Categories
Funding
- National Institutes of Health [R01GM49500, P30 ES020957, P30 CA 022453, S10 OD010700]
- National Cancer Institute [RO1CA163895, P5OCA130810, R21CA189775]
Ask authors/readers for more resources
Pancreatic cancer is the third leading cause of cancer-related death in the USA: Despite extensive research, minimal improvements in patient outcomes have been achieved. Early identification of treatment response and metastasis would be valuable to determine the appropriate therapeutic course for patients. In this work, we isolated exosomes from the serum of 10 patients with locally advanced pancreatic cancer at serial time points over a course of therapy, and quantitative analysis was performed using the iTRAQ method. We detected approximately 700-800 exosomal proteins per sample, several of which have been implicated in metastasis and treatment resistance. We compared the exosomal proteome of patients at different time points during treatment to healthy controls and identified eight proteins that show global treatment-specific changes. We then tested the effect of patient-derived 'exosomes on the migration of tumor cells and found that patient-derived exosomes, but not healthy controls; induce cell migration, supporting their role in metastasis. Our data Show that exosomes can be reliably extracted from patient serum and analyzed for protein content. The differential loading of exosomes during a course of therapy suggests that exosomes may provide novel insights into the development Of treatment resistance and metastasis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available