Journal
JOURNAL OF PROTEOME RESEARCH
Volume 16, Issue 3, Pages 1193-1206Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.6b00720
Keywords
Ustilaginoidea virens; protein-protein interaction (PPI); interolog; domain-domain interaction; pathogenicity; secreted protein
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Funding
- National Natural Science Foundation of China [31630064, 31471728]
- Key Projects in the National Science & Technology Pillar Program [2012BAD19B03]
- 111 project [B13006]
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Rice false smut, caused by Ustilaginoidea virens, produces significant losses in rice yield and grain quality and has recently emerged as one of the most important rice diseases worldwide. Despite its importance in rice production, relatively few studies have been conducted to illustrate the complex interactome and the pathogenicity gene interactions. Here a protein protein interaction network of U. virens was built through two well-recognized approaches, interolog- and domain domain interaction-based methods. A total of 20 217 interactions associated with 3305 proteins were predicted after strict filtering. The reliability of the network was assessed computationally and experimentally. The topology of the interactome network revealed highly connected proteins. A pathogenicity-related subnetwork involving up-regulated genes during early U. virens infection many novel pathogenicity proteins were predicted in the subnetwork. In addition, we built an interspecies PPI network between U. virens and Oryza sativa, providing new insights for molecular interactions of this host pathogen pathosystem. A web-based publicly available interactive database based on these interaction networks has also been released. In summary, a proteome-scale map of the PPI network was described for U. virens, which will provide new perspectives for finely dissecting interactions of genes related to its pathogenicity.
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