Journal
JOURNAL OF PROTEOME RESEARCH
Volume 16, Issue 12, Pages 4391-4402Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.7b00391
Keywords
Chromosome-Centric Human Proteome Project (C-HPP); Y chromosome; human embryonic stem cell; cardiac differentiation; TBL1Y
Categories
Funding
- Royan Institute
- Iran Science Elites Federation
- Iranian National Science Foundation (INSF)
Ask authors/readers for more resources
Despite evidence for sex-specific cardiovascular physiology and pathophysiology, the biological basis for this dimorphism remains to be explored. Apart from hormonal factors, gender-related characteristics may reside in the function of sex chromosomes during cardiac development. In this study, we investigated the differential expression of the male-specific region of the Y chromosome (MSY) genes and their X counterparts during cardiac differentiation of human embryonic stem cells (hESC). We observed alterations in mRNA and protein levels of TBL1Y, PCDHI1Y, ZFY, KDMSD, USP9Y, RPS4Y1, DDX3Y, PRY, XKRY, BCORPI, RBMY, HSFY, and UTY, which accompanied changes in intracellular localization. Of them, the abundance of a Y chromosome missing protein, TBL1Y, showed a significant increase during differentiation while the expression level of its X counterpart decreased. Consistently, reducing TBL1Y cellular level using siRNA approach influenced cardiac differentiation by reducing its efficacy as well as increasing the probability of impaired contractions. TBL1Y knockdown may have negatively impacted cardiogenesis by CtBP stabilization. Furthermore, we presented compelling experimental evidence to distinguish TBL1Y from TBL1X, its highly similar X chromosome homologue, and proposed reclassification of TBL1Y as found missing protein (PE1). Our results demonstrated that MSY proteins may play an important role in cardiac development.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available