Journal
JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE
Volume 180, Issue 6, Pages 788-799Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jpln.201700013
Keywords
gene transcription; NO; Oryza sativa L; selenite
Categories
Funding
- National Key Research and Development Program of China [2017YFC0506102]
- Natural Science Foundation of China (NSFC) [31260057, 31570586, 31300505]
- State Key Laboratory of Soil and Sustainable Agriculture, Nanjing Institute of Soil Science, the Chinese Academy of Science [Y412201449]
- China Postdoctoral Science Foundation [2012 M521278]
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Nitric oxide (NO) plays a key role in regulating a wide range of physiological and biochemical processes. This study investigated possible effects of NO, which was supplied by sodium nitroprusside (SNP), NO donor, on selenium (Se) concentration, Se species within roots, activities and gene transcription of selenite uptake, and metabolism-related transporters or enzymes using ICP-MS, HPLC-ICP/MS, qRT-PCR, fluorescence imaging, and biochemical analysis in the roots of rice (Oryza sativa L.) seedling. A suitable SNP concentration (10 mu M) increased Se concentration in rice roots. NO enhanced the gene transcription of phosphate transporter OsPT2 and sulfate transporters OsSultr1;2 and OsSultr4;1 that contribute to selenite uptake. Furthermore, NO increased selenocysteine and methyl-selenocysteine concentrations in roots, stimulated glutathione biosynthesis by up-regulating the transcript levels of -glutamylcysteine synthetase (-ECS) and glutathione synthetase (GS), and increased cysteine synthase (CS) activity. In conclusion, our results indicate that NO promotes Se concentration of rice seedling roots by enhancing selenite uptake, up-regulating gene transcription of selenite uptake-related transporters, and increasing Se metabolism through regulating glutathione biosynthesis.
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