Production of Host-selective SV-toxins by Stemphylium sp Causing Brown Spot of European Pear in Japan

Recently, a new disease known as brown spot of European pear' caused by Stemphylium sp. appeared on the leaves, twigs and fruits of the cultivar Le Lectier in Niigata Prefecture, Japan. Because Stemphylium vesicarium (teleomorph: Pleospora allii), which causes a similar disease in Europe, has been shown to produce host-selective SV-toxins in culture filtrates (CFs), SV-toxin production by Stemphylium sp. in Japan was investigated. In pathogenicity tests, the pathogen induced severe necrotic spots on the leaves of the European pear cultivar Le Lectier, slight spots on cultivar La France and slight or no spots on cultivar Bartlett. The Japanese pear cultivar Nijisseiki was not affected by the pathogen. Culture filtrates of the pathogen were tested for phytotoxicity on cultivars by a leaf necrosis assay. The sensitivity of cultivars to the CFs was consistent with the susceptibility of cultivars to the pathogen infection, indicating the presence of host-selective toxins. The toxins were purified from the CFs according to the procedure reported for SV-toxin purification in S.vesicarium. The results indicated that Stemphylium sp. in Japan produces the same SV-toxins as S.vesicarium in Europe.