Journal
BASIC AND CLINICAL NEUROSCIENCE
Volume 10, Issue 4, Pages 373-381Publisher
IRAN UNIV MEDICAL SCIENCES
DOI: 10.32598/bcn.9.10.130
Keywords
Reward; Orexin system; Reinstatement; Ventral tegmental area; Forced swim stress; Conditioned place preference
Categories
Funding
- Sanandaj Branch, Islamic Azad University, Sanandaj [1109610020017]
Ask authors/readers for more resources
Introduction: Orexin-containing neurons exist in the lateral hypothalamic region, sending their projections toward mesolimbic regions such as the Ventral Tegmental Area (VTA). Methods: In the current study, a Reinstatement model is used to examine the effects of intra-VTA administration of SB334867 as an Orexin-1 Receptor (OX1R) antagonist on drug priming- and Forced Swim Stress (FSS)-induced reinstatement of morphine. Eighty-eight male adult albino Wistar rats, weighing 200-280 g, were bilaterally implanted by cannulas into the VTA. We induced the Conditioned Place Preference (CPP) by Subcutaneous (SC) injection of morphine (5 mg/kg) daily in three days. Then, the CPP score was calculated. After a 24-h off period following achievement of extinction criterion, the rats were tested for drug priminginduced reinstatement by a priming dose of morphine (1 mg/kg, SC) and for FSS-induced reinstatement 10 min after FSS. In the next experiments, the animals received different doses of intra-VTA administration of SB334867 (0.3, 3, and 1 nM/0.3 mu L 12% DMSO per side) and bilaterally were subsequently tested for FSS- and morphine priming-induced reinstatement. Results: Our findings indicated that the FSS could induce the reinstatement of seeking behaviors. Furthermore, intra-VTA administration of OX1R antagonists suppressed FSS- and drug priming-induced reinstatement dose-dependently. Conclusion: It is concluded that FSS and drug priming-induced reinstatement might be mediated, at least in part, by Stimulation of orexin receptors in the VTA.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available