Journal
JOURNAL OF PHYSICAL CHEMISTRY C
Volume 121, Issue 46, Pages 25994-25999Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jpcc.7b10549
Keywords
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Funding
- Russian Science Foundation [17-73-20030]
- NIH [1R21EB018014, 1R21EB020323, 1R21CA220137]
- NSF [CHE-1416268, CHE-1416432]
- DOD [CDMRP W81XWH-12-1-0159/BC112431, W81XWH-15-1-0271, W81XWH-15-1-0272]
- SIUC MTC
- OSPA
- Haldor Topsoe A/S
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1836308, 1416432] Funding Source: National Science Foundation
- Russian Science Foundation [17-73-20030] Funding Source: Russian Science Foundation
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The signal amplification by reversible exchange (SABRE) technique employs exchange with singlet-state parahydrogen to efficiently generate high levels of nuclear spin polarization. Spontaneous SABRE has been shown previously to be efficient in the milli-Tesla and micro-Tesla regimes. We have recently demonstrated that high-field SABRE is also possible, where proton sites of molecules that are able to reversibly coordinate to a metal center can be hyperpolarized directly within high field magnets, potentially offering the convenience of in situ hyper-polarization-based spectroscopy and imaging without sample shuttling. Here, we show efficient polarization transfer from parahydrogen (para-H-2) to the N-15 atoms of imidazole-N-15(2) and nicotinamide-N-15 achieved via high-field SABRE (HF-SABRE). Spontaneous transfer of spin order from the para-H-2 protons to N-15 atoms at the high magnetic field of an MRI scanner allows one not only to record enhanced N-15 NMR spectra of in situ hyperpolarized biomolecules but also to perform imaging using conventional MRI sequences. 2D N-15 MRI of high-field SABRE-hyperpolarized imidazole with spatial resolution of 0.3 x 0.3 mm(2) at 9.4 T magnetic field and a high signal-to-noise ratio (SNR) of similar to 99 was demonstrated. We show that H-1 MRI of in situ HF-SABRE hyperpolarized biomolecules (e.g., imidazole-N-15(2)) is also feasible. Taken together, these results show that heteronuclear (N-15) and H-1 spectroscopic detection and imaging of high-field-SABRE-hyperpolarized molecules are promising tools for a number of emerging applications.
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