Journal
NEW JOURNAL OF CHEMISTRY
Volume 43, Issue 37, Pages 14763-14771Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9nj03375k
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Funding
- Key Science and Technology Programme of Henan Province [182102310101]
- National Natural Science Foundation of China [21807027, 81130062]
- Fundamental Research Funds for the Provincial Universities [2014KYYWF-QN04]
- Graduate Innovation Fund in Henan College of Chinese Medicine [YJS2018A05]
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Cysteine, as an important amino acid in the human body, plays a vital role in people's normal life activities. In this paper, a ratiometric fluorescent probe for detecting cysteine was designed and synthesized based on the fluorescence resonance energy transfer (FRET) process. In this FRET system, a coumarin derivative was used as the energy donor, a rhodol fluorophore was chosen as the energy receptor, and an acrylate group was utilized as a cysteine recognition unit. In the absence of cysteine, the rhodol receptor was in the non-fluorescent lactone state and the FRET process was inhibited. Upon addition of cysteine, the closed spirolactone form was converted to a conjugated fluorescent xanthene form to induce the occurrence of FRET which resulted in a fluorescent signal decrease at 470 nm and enhancement at 543 nm. The ratiometric fluorescent probe exhibited excellent selectivity to Cys over Hcy and GSH. In addition, I-543nm/I-470nm of the probe for cysteine displayed a good linear relationship in the range of 5.0 x 10(-7)-1.0 x 10(-4) mol L-1, and the detection limit was 2.0 x 10(-7) mol L-1. Furthermore, the probe showed low cell toxicity and was successfully applied to the confocal imaging of cysteine in HepG2 cells using dual emission channels.
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