Journal
JOURNAL OF PHYSICAL CHEMISTRY B
Volume 121, Issue 9, Pages 2121-2126Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jpcb.6b10949
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Funding
- Oklahoma Centre for the Advancement of Science and Technology [HR 12-128]
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Easy to use and easy to produce biosensors would have.a huge range of applications. To reach this goal many see the incorporation of a protein into a sol gel network as one of the most viable options. The current most prevalent technique of predoping presents inherent limits on the concentration possible for the resulting thin film. In this study we demonstrate a new process utilizing the newly developed kinetic doping method to load silica sol gel thin films with cytochrome C (CytC) and horseradish peroxidase (HRP). Both enzymes are shown to successfully load and have a concentration increase over their original loading solution by factors t.T, v co U c of 1300X and 2600X, respectively. Furthermore, each enzyme once loaded retained the ability to act as a catalyst for the detection of hydrogen peroxide. Ultimately the CytC- and HRP-loaded thin films were found to have enzyme concentrations of 11 1 mM and 6.0 +/- 0.4 mM, respectively, a considerable step up from any doping method reported in the
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