Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
Volume 338, Issue -, Pages 178-182Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotochem.2017.02.008
Keywords
Fluorescent probe; Cysteine; Cells imaging; Coumarin
Categories
Funding
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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Herein, we report a novel fluorescent probe based on coumarin to detect cysteine (Cys). The probe can react rapidly and selectively with Cys but not with the other amino acids. When the Cys was added to the solution of the probe, we could observe obvious color change from pale green to bright green. The detection limit of this probe for Cys was found to be 0.06 mu M. Additionally, bioimaging of intracellular Cys by this probe was successfully applied in living cell's, which indicating that this probe holds great potential for biological applications. (C) 2017 Elsevier B.V. All rights reserved.
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