Journal
JOURNAL OF PHARMACEUTICAL SCIENCES
Volume 106, Issue 1, Pages 377-384Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.xphs.2016.06.028
Keywords
alkyd nanoemulsion; phenytoin; topical; wound healing
Funding
- International Medical University [IMU272/2013]
- Malaysia Toray Science Foundation (MTSF) Science and Technology [IMUR160/2014]
Ask authors/readers for more resources
Phenytoin-loaded alkyd nanoemulsions were prepared spontaneously using the phase inversion method from a mixture of novel biosourced alkyds and Tween 80 surfactant. Exposure of human adult keratinocytes (HaCaT cells) for 48 h to alkyd nanoemulsions producing phenytoin concentrations of 3.125-200 mu g/mL resulted in relative cell viability readings using tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide of 100% confirming nontoxicity and suggesting cell proliferation activity. Phenytoin-loaded alkyd nanoemulsions generally resulted in higher mean cell viability compared with equivalent concentration of phenytoin solutions, suggesting that the nanoemulsions provided a controlled-release property that maintained the optimum phenytoin level for keratinocyte growth. HaCaT cell proliferation, measured by 5-bromo-2-deoxyuridine uptake, was found to increase following exposure to increasing phenytoin concentration from 25 to 50 mg/mL in solution or encapsulated in nanoemulsions but declined at a drug concentration of 100 mg/mL. An in vitro cell monolayer wound scratch assay revealed that phenytoin solution or nanoemulsions producing 50 mg/mL phenytoin concentration resulted in 75%-82% scratch closure after 36 h, similar to medium containing 10% fetal bovine serum as a cell growth promoter. These findings indicate that phenytoin-loaded alkyd nanoemulsions show potential for promoting topical wound healing through enhanced proliferation of epidermal cells. (C) 2016 American Pharmacists Association (R). Published by Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available