AMELIORATION WITH TITANIUM DIOXIDE NANOPARTICLE FOR REGULATION OF OXIDATIVE STRESS IN MAIZE (Zea mays L.)

In the present experiment, Zea mays L. was evaluated for its C-4 modality through oxidative stress responses. Moreover, under variable concentrations (0, 50, 100 mu M) of hydrogen peroxide, the impacts were variable and modulated with titanium dioxide nanoparticle (TiO2-NP). Plants scored with elevated concentrations of H2O2 and superoxide (O-2(center dot-)) maximum by 1.31 & 1.52-fold respectively over 0 mu M H2O2. TiO2-NP recovered those maximums with 8.69 & 7.89% as compared to control plants. The effects of H2O2 were moderated with malondialdehyde and carbonyl content by peak values 7.45 & 5.91% under TiO2-NP treatment respectively. A depletion in proline content recorded under H2O2 stress but recovered through highest 1.23-fold with TiO2-NP application. TiO2-NP recorded NADP-ME activities up-regulated in concentration gradient of H2O2. Oxidative exposure was also documented by in-vivo detection of H2O2 and O-2(center dot-) by 3,3'-diaminobenzidine and nitroblue tetrazolium staining. For the anti-oxidative cascades superoxide dismutase activity had induced by 1.42-fold under H2O2 when pre-treated with TiO2-NP. However, glutathione reductase activity was differentially modulated under H2O2 through interaction with TiO2-NP. Activity of guaiacol peroxidase was significantly up-regulated by 1.07-fold when TiO2-NP applied. The inductions of oxidative stress recorded more evident when a distinct polymorphism for GPX protein resolved variations in band intensities, but not in number. Activity of catalase experience a down-regulated trend all through the H2O2 exposure and had significant sensitivity to TiO2-NP. The results depict the nature and reactivity of C-4 modules under oxidative stress that would be the selection pressures and that might be modulated with TiO2-NP application