Journal
JOURNAL OF PEPTIDE SCIENCE
Volume 23, Issue 7-8, Pages 631-635Publisher
WILEY
DOI: 10.1002/psc.2980
Keywords
sortase A; sortase-mediated ligation; phage display; protein engineering; directed evolution
Funding
- Deutsche Forschungsgemeinschaft [SPP 1623, SCHW 1163/7-1, FR 1325/15-1]
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Sortase-mediated ligation is one of the most commonly used chemo-enzymatic techniques for the site-specific modification of proteins. We have established a new library of sortase mutants for directed evolution of sortase substrate selectivity. Phage display screens of this second-generation library yielded sortase mutants that ligate substrate proteins containing an APxTG or FPxTG recognition sequence instead of the canonical LPxTG sorting motif. These findings indicate that the second-generation sortase library is well suited for sortase engineering in order to increase the versatility of sortase-mediated ligation. Copyright (c) 2017 European Peptide Society and John Wiley & Sons, Ltd.
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