Journal
NATURE PLANTS
Volume 5, Issue 10, Pages 1033-1042Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41477-019-0522-9
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Funding
- Finnish Centre of Excellence in Molecular Biology of Primary Producers (Academy of Finland CoE programme) [271832]
- Gatsby Foundation [GAT3395/PR3]
- University of Helsinki [799992091]
- European Research Council Advanced Investigator Grant SYMDEV [323052]
- Academy of Finland [271832, 132376, 266431]
- University of Helsinki HiLIFE fellowship
- National Research Foundation of Korea [2018R1A5A1023599, 2016R1A2B2015258]
- National Research Foundation of Korea [2016R1A2B2015258] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Academy of Finland (AKA) [266431, 132376, 266431, 132376] Funding Source: Academy of Finland (AKA)
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Vascular cambium, a lateral plant meristem, is a central producer of woody biomass. Although a few transcription factors have been shown to regulate cambial activity', the phenotypes of the corresponding loss-of-function mutants are relatively modest, highlighting our limited understanding of the underlying transcriptional regulation. Here, we use cambium cell-specific transcript profiling followed by a combination of transcription factor network and genetic analyses to identify 62 new transcription factor genotypes displaying an array of cambial phenotypes. This approach culminated in virtual loss of cambial activity when both WUSCHEL-RELATED HOMEOBOX 4 (WOX4) and KNOTTED-like from Arabidopsis thaliana 1 (KNAT1; also known as BREVIPEDICELLUS) were mutated, thereby unlocking the genetic redundancy in the regulation of cambium development. We also identified transcription factors with dual functions in cambial cell proliferation and xylem differentiation, including WOX4, SHORT VEGETATIVE PHASE (SVP) and PETAL LOSS (PTL). Using the transcription factor network information, we combined overexpression of the cambial activator WOX4 and removal of the putative inhibitor PTL to engineer Arabidopsis for enhanced radial growth. This line also showed ectopic cambial activity, thus further highlighting the central roles of WOX4 and PTL in cambium development.
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