Journal
JOURNAL OF ORGANOMETALLIC CHEMISTRY
Volume 827, Issue -, Pages 67-77Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jorganchem.2016.11.007
Keywords
Cu(II) complexes; Calf thymus DNA; Bovine serum albumin; Comet assay; MCF-7; HEK-293T
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Funding
- VEGA [1/0001/13, 0752/13]
- UHHK [00179906]
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This study examined a two of Cu(II) complexes containing Schiff bases [Cu(salglu)Meim] (1) and [Cu(et)(2)(im)(4)][Cu-2(salglu)(2)(im)(2)] (2), where salglu = (N-salicylidene-D,L-glutamato)(2-) Meim = 2-methylimidazole, im = imidazole and et = ethanol. The interactions and binding characteristics of the complexes with calf thymus DNA (ctDNA) and bovine serum albumin (BSA) were investigated using UV-Vis and fluorescence spectroscopy. The binding constants K were calculated from the UV-Vis spectroscopic titration results using the McGhee and Hippel equation, and were found to be K = 8.9 (for 1) and 3.1 x 10(3) M-1 (for 2) in the case of complex-DNA interactions, while Stern-Volmer quenching results were used to determine the values K = 2.74 x 10(5) and 2.54 x 10(6) M-1 (for 1 and 2, respectively) in the case of complex-BSA binding. DNA strand breaks in cellular DNA were investigated using single cell gel electrophoresis (comet assay). MCF-7 cancer cell lines and healthy HEK-293T cell lines were exposed to complexes 1 and 2 at a concentration range of 0.001-100 x 10(-6) M for 24 h. Complexes 1 and 2 induced significant DNA damage in MCF-7 cancer cell lines, while healthy HEK-293T cell lines were more resistant against their effect. (C) 2016 Elsevier B.V. All rights reserved.
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