Journal
JOURNAL OF ORAL PATHOLOGY & MEDICINE
Volume 47, Issue 2, Pages 144-151Publisher
WILEY
DOI: 10.1111/jop.12654
Keywords
cell junction; invasion; metastasis; salivary adenoid cystic carcinoma; tumor-derived exosomes
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Funding
- Natural Science Foundation of Guangdong Province, China [2016A030313590]
- Science and Technology Planning Project of Guangdong Province, China [2014A020212397]
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ObjectivesTumor-derived exosomes (TDE) have been shown to participate in different steps of the dissemination of cancer cells. However, the role of salivary adenoid cystic carcinoma-derived (SACC-derived) exosomes had not been documented in SACC. The study aims to explore the functions of SACC-derived TDE in SACC progression and investigate potential mechanisms. MethodsSalivary adenoid cystic carcinoma cell line SACC-83 was used to generate TDE. Afterward, SACC-83 or HUVECs were cocultured with or without TDE. Tumor migration, tumor invasion, and endothelial permeability were examined by wound healing assay, tumor invasion assay, endothelial permeability assay, and tumor cell transendothelial migration assay, respectively. Moreover, the expression levels of cell junction-related proteins were examined by qRT-PCR and Western blot. ResultsSalivary adenoid cystic carcinoma -83-derived exosomes were taken up by their host cells. Meanwhile, TDE increased migration and invasion capacity of SACC-83 cells and enhanced endothelial cell permeability. Furthermore, we demonstrated that the expression of cell junction-related proteins (Claudins and ZO-1) was downregulated, whichis presumablyinvolved in the TDE-mediated promotion of migration, invasion, and metastasis. ConclusionThe results suggested that SACC cell-derived exosomes were loaded with individual components that could enhance invasiveness and induce microenvironment changes, thus promoting SACC aggression.
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