Journal
BIOMATERIALS SCIENCE
Volume 7, Issue 11, Pages 4536-4546Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9bm01285k
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [31501896]
- Chongqing Science and Technology Commission [cstc2018jcyjAX0298]
- Fundamental Research Funds for the Central Universities [XDJK2018C064]
- Chongqing Engineering and Technology Research Center for Novel Silk Materials [SILKGCZX2016002]
Ask authors/readers for more resources
Functional silk mats with improved cell proliferation activity are promising medical materials to accelerate damaged wound healing and tissue repair. In this study, novel functional silk mats were fabricated from human fibroblast growth factor (FGF)-containing cocoons generated by expressing human acid FGF1 and basic FGF2 in silkworms. First, functional silk mats containing FGF1 and FGF2 proteins alone or in combination were fabricated by physically cutting genetically engineered cocoons. Compared to those of normal silk mats, the physical properties of these functional silk mats such as silk fibre diameter, internal secondary structure, and mechanical properties were significantly changed. The expressed FGF1 and FGF2 proteins in these silk mats were efficiently and gradually released over 15 days. Moreover, these silk mats significantly promoted NIH/3T3 cell proliferation and growth by activating the extracellular signalregulated kinase (ERK) pathway, and the silk mat containing FGF1 and FGF2 proteins showed higher cell proliferation. Importantly, this silk mat caused no obvious cytotoxicity or cell inflammation. These results suggest that these functional silk mats have potential medical applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available