Journal
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
Volume 44, Issue -, Pages 44-51Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jnutbio.2017.02.018
Keywords
Ferulic acid; Ferulic acid-4-O-sulfate; Mouse; Vasorelaxation; Blood pressure
Funding
- Agency for Innovation by Science and Technology in Flanders (IWT)
- European Union Seventh Framework Programme [FP7] [312090]
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Consumption of foods rich in ferulic acid (FA) such as wholegrain cereals, or FA precursors such as chlorogenic acids in coffee, is inversely correlated with risk of cardiovascular disease and type 2 diabetes. As a result of digestion and phase II metabolism in the gut and liver, FA is converted predominantly into ferulic acid-4-0-sulfate (FA-sul), an abundant plasma metabolite. Although FA-sul is the main metabolite, very little has been reported regarding its bioactivities. We have compared the ex vivo vasorelaxing effect of FA and FA-sul (10(-7)-3.10(-5) M) on isolated mouse arteries mounted in tissue myographs. FA-sul, but not FA, elicited a concentration-dependent vasorelaxation of saphenous and femoral arteries and aortae. The FA-sul-mediated vasorelaxation was blunted by 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, a soluble guanylate cyclase (sGC) inhibitor. The role of sGC was confirmed in femoral arteries isolated from sGC alpha((-/-))(1) knockout mice. Furthermore, 4-aminopyridine, a specific inhibitor of voltage-dependent potassium channels, significantly decreased FA-sul-mediated effects. In anesthetized mice, intravenous injection of FA-sul decreased mean arterial pressure, whereas FA had no effect, confirming the results obtained ex vivo. FA-sul is probably one of the major metabolites accounting for the blood pressure-lowering effects associated with FA consumption. (C) 2017 Elsevier Inc. All rights reserved.
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