Journal
JOURNAL OF NEUROSCIENCE RESEARCH
Volume 95, Issue 11, Pages 2297-2306Publisher
WILEY
DOI: 10.1002/jnr.24032
Keywords
glucose; metabolism; MRS; thiopental; alpha-chloralose; neuron-glia interaction
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Funding
- Swiss National Science Foundation [148250, 149983]
- National Competence Center in Biomedical Imaging (NCCBI)
- Centre d'Imagerie BioMedicale (CIBM) of the UNIL
- UNIGE
- HUG
- CHUV
- EPFL
- Leenaards Foundation
- Jeantet Foundation
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Barbiturates, commonly used as general anaesthetics, depress neuronal activity and thus cerebral metabolism. Moreover, they are likely to disrupt the metabolic support of astrocytes to neurons, as well as the uptake of nutrients from circulation. By employing C-13 magnetic resonance spectroscopy (MRS) in vivo at high magnetic field, we characterized neuronal and astrocytic pathways of energy metabolism in the rat cortex under thiopental anaesthesia. The neuronal tricarboxylic acid (TCA) cycle rate was 0.46+/-0.02mmol/g/min, and the rate of the glutamate-glutamine cycle was 0.09+/-0.02mmol/g/min. In astrocytes, the TCA cycle rate was 0.16+/-0.02mmol/g/min, accounting for a quarter of whole brain glucose oxidation, pyruvate carboxylase rate was 0.02+/-0.01mmol/g/min, and glutamine synthetase was 0.12+/-0.01mmol/g/min. Relative to previous experiments under light a-chloralose anaesthesia, thiopental reduced oxidative metabolism in neurons and even more so in astrocytes. Interestingly, total oxidative metabolism in the cortex under thiopental anaesthesia surpassed the rate of pyruvate production by glycolysis, indicating substantial utilisation of substrates other than glucose, likely plasma lactate. (C) 2017 Wiley Periodicals, Inc.
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