Journal
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Volume 82, Issue 1, Pages 49-53Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2015.01.009
Keywords
Drug susceptibility testing; Multidrug-resistant tuberculosis; Level of resistance; Mutations
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Funding
- National Health and Education Society, Hinduja Hospital, and Medical Research Centre
- National Institute of Allergy and Infectious Diseases grant [U01-AI082229, R01AI111435-04]
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This study correlates MICs of rifampicin (RIF) and isoniazid (INH) with GenoType MTBDRplus assay results for drug-resistant Mycobacterium tuberculosis (MTB) clinical isolates. MICs of RIF and INH were established for 84 and 90 isolates, respectively, testing 7 concentrations of each drug. Genotypic resistance to each drug was determined by GenoType MTBDRplus assay with 50 representative mutations confirmed by pyrosequencing, with mutations in the rpoB gene associated with RIF resistance and mutations in the katG and/or inhA genes associated with INH resistance. Based upon the correlation of MICs with specific genetic profiles, relative resistance levels were established for each isolate. Results indicate that MTB phenotypic resistance, currently based upon the testing of isolate susceptibility to a single drug concentration, may be more accurately profiled via quantitative MICs, and therefore, the correlation of molecular diagnostic results with specific MICs may allow for more optimal treatment of infections. (C) 2015 Elsevier Inc. All rights reserved.
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