Journal
JOURNAL OF NEUROSCIENCE
Volume 37, Issue 10, Pages 2723-2733Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.3377-16.2017
Keywords
fiberphotometry; food-incentive goal-directed behavior; motivation; optogenetic inhibition; ventrolateral striatum; yellow cameleon
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Funding
- Japan Society for the Promotion of Science Grant for Research Fellow [15J06790, 2640100]
- Takeda Science Foundation
- [15H03123]
- [16H01621]
- Grants-in-Aid for Scientific Research [25117005, 15J06790, 15KT0111, 17H06062, 16K07032, 15K06732, 16H01620] Funding Source: KAKEN
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The ventral striatum is involved in motivated behavior. Akin to the dorsal striatum, the ventral striatum contains two parallel pathways: the striatomesencephalic pathway consisting of dopamine receptor Type 1-expressing medium spiny neurons (D1-MSNs) and the striatopallidal pathway consisting of D2-MSNs. These two genetically identified pathways are thought to encode opposing functions in motivated behavior. It has also been reported that D1/D2 genetic selectivity is not attributed to the anatomical discrimination of two pathways. We wanted to determine whether D1-and D2-MSNs in the ventral striatum functioned in an opposing manner as previous observations claimed, and whether D1/D2 selectivity corresponded to a functional segregation in motivated behavior of mice. To address this question, we focused on the lateral portion of ventral striatum as a region implicated in food-incentive, goal-directed behavior, and recorded D1 or D2-MSN activity by using a gene-encoded ratiometric Ca2+ indicator and by constructing a fiberphotometry system, and manipulated their activities via optogenetic inhibition during ongoing behaviors. We observed concurrent event-related compoundCa(2+) elevations in ventrolateral D1-and D2-MSNs, especially at trial start cue-related and first lever press-related times. D1 or D2 selective optogenetic inhibition just after the trial start cue resulted in a reduction of goal-directed behavior, indicating a shared coding of motivated behavior by both populations at this time. Only D1-selective inhibition just after the first lever press resulted in the reduction of behavior, indicating D1-MSN-specific coding at that specific time. Our data did not support opposing encoding by both populations in food-incentive, goal-directed behavior.
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