Journal
JOURNAL OF NEUROSCIENCE
Volume 37, Issue 43, Pages 10258-10277Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.2085-17.2017
Keywords
axotomy; macrophages; neutrophils; phagocytosis; sciatic nerve; Wallerian degeneration
Categories
Funding
- National Institutes of Health [DK097223, NS095017]
- Office of Research Infrastructure Shared Instrumentation Grant [S10OD016164]
- [NS067431]
- [F31NS093694]
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Wallerian degeneration (WD) is considered an essential preparatory stage to the process of axonal regeneration. In the peripheral nervous system, infiltrating monocyte-derived macrophages, which use the chemokine receptor CCR2 to gain entry to injured tissues from the bloodstream, are purportedly necessary for efficient WD. However, our laboratory has previously reported that myelin clearance in the injured sciatic nerve proceeds unhindered in the Ccr2(-/-) mouse model. Here, we extensively characterize WD in male Ccr2(-/-) mice and identify a compensatory mechanism of WD that is facilitated primarily by neutrophils. In response to the loss of CCR2, injured Ccr2(-/-) sciatic nerves demonstrate prolonged expression of neutrophil chemokines, a concomitant extended increase in the accumulation of neutrophils in the nerve, and elevated phagocytosis by neutrophils. Neutrophil depletion substantially inhibits myelin clearance after nerve injury in both male WT and Ccr2(-/-) mice, highlighting a novel role for these cells in peripheral nerve degeneration that spans genotypes.
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