Journal
JOURNAL OF NEUROINFLAMMATION
Volume 14, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s12974-017-0860-3
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Funding
- National Institute of Mental Health [MH-066703]
- National Institute of Neurological Disorders and Stroke [NS-038836]
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Background: Previous work from our laboratory has demonstrated that during acute viral brain infection, glial cells modulate antiviral T cell effector responses through the PD-1: PD-L1 pathway, thereby limiting the deleterious consequences of unrestrained neuroinflammation. Here, we evaluated the PD-1: PD-L1 pathway in development of brain-resident memory T cells (bT(RM)) following murine cytomegalovirus (MCMV) infection. Methods: Flow cytometric analysis of immune cells was performed at 7, 14, and 30 days post-infection (dpi) to assess the shift of brain-infiltrating CD8(+) T cell populations from short-lived effector cells (SLEC) to memory precursor effector cells (MPEC), as well as generation of bT(RMs). Results: In wild-type (WT) animals, we observed a switch in the phenotype of brain-infiltrating CD8(+) T cell populations from KLRG1(+) CD127(-) (SLEC) to KLRG1(-) CD127(+) (MPEC) during transition from acute through chronic phases of infection. At 14 and 30 dpi, the majority of CD8(+) T cells expressed CD127, a marker of memory cells. In contrast, fewer CD8(+) T cells expressed CD127 within brains of infected, PD-L1 knockout (KO) animals. Notably, in WT mice, a large population of CD8(+) T cells was phenotyped as CD103(+) CD69(+), markers of bTRM, and differences were observed in the numbers of these cells when compared to PD-L1 KOs. Immunohistoche mical studies revealed that brain-resident CD103(+) bT(RM) cells were localized to the parenchyma. Higher frequencies of CXCR3 were also observed among WT animals in contrast to PD-L1 KOs. Conclusions: Taken together, our results indicate that bT(RMs) are present within the CNS following viral infection and the PD-1: PD-L1 pathway plays a role in the generation of this brain-resident population.
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