Journal
ACS APPLIED BIO MATERIALS
Volume 2, Issue 11, Pages 4911-4921Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsabm.9b00661
Keywords
poly(HEMA); differentiation; adhesion; surface patterning; hydrogel
Funding
- Oklahoma State University
- NSFCAREER [CHE-1554924]
- NSF MRI program
- IPRMO (OSU)
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Micropillar patterns were fabricated and used to study cell adhesion, morphology, and function. Micropillars were produced in poly(2-hydroxyethyl methacrylate (HEMA)/N,N-(dimethylaminoethyl)methacrylate (DMAEMA)/tetraethylene glycol dimethacrylate (TEGDMA)) hydrogels using soft lithography, had dimensions of 1 mu m diameter, and were either 2.05 or 4.91 mu m tall. The patterned hydrogel substrates increased adhesion and induced the formation of cellular aggregates. Digital micrographs were used to quantify aggregate size and number. Differentiation of hMSCs toward adipocytes and chondrocytes was performed using the respective complete culture and differentiation medium for 2 weeks. Cells were stained for Oil red O, Alcian blue, and Type II collagen. Hydrogel substrates supported the differentiation of hMSCs to adipocytes and chondrocytes. The taller micropillar patterns supported the attachment and growth of larger aggregates and were more amenable to aid chondrogenic differentiation.
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