A Facile and Universal Route to Mass Yield Multifunctional Janus Nanoparticles and Their Sensing of a Tumor Biomarker

The controlled material distribution is important for creating anisotropic building blocks and introduces an extra design parameter. Recently, Janus particles have garneredmuch interest due to their anisotropic properties for many applications in research and technology. In this paper, we reported a one-step-two-phase emulsion synthesis approach as a universal route to prepare Janus nanoparticles with multifunctional (like plasmonic, magnetic and fluorescent) properties. By using magnetic and fluorescent Janus nanoparticles to replace the fixed primary capture antibodies on microtiter plates and enzyme-linked secondary antibodies for colorimetric detection, respectively, the in vitro detection of prostate specific agents conducted under the consideration of simplifying the traditional enzyme-linked immune sorbent assay, and the linear concentration-dependent detection range can fit for the current clinical diagnostic requirements. We hope this mass yield multifunctional Janus nanoparticles' method could shed light to those fields of both traditional colorimetric biosensing and modern precision medicine.