Acalypha wilkesiana flowers: Phenolic profiling, cytotoxic activity of their biosynthesized silver nanoparticles and molecular docking study for its constituents as Topoisomerase-I inhibitors

The bio potential behavior of green silver nanoparticles (AgNPs) using Acalypha wilkesiana flowers extract weas evaluated. The AgNPs formed were confirmed using surface plasmon resonance band showed in UV-vis spectrophotometer that characterized with spherical shape in size range from 5 to 45 nm depending on Transmission electron microscopy (TEM) and with good stability ( - 50.75 mV) depending on Zeta-potential measurements. In order to identify the capping agents responsible for the reduction of silver ion and the stabilization of nanoparticles prepared, Fourier transform-infrared spectroscopy (FTIR) and HPLC-ESI-MS analysis were used. Our AgNPs exhibited cytotoxic activity against MCF-7 (Breast carcinoma), PC-3 (Prostate carcinoma) and BJ-1 (skin normal human) cell lines with IC50 values 4.00, 3.6 and 4.1 mu g/mL, respectively (i.e. the higher selectivity of AgNPs toward prostate than breast cancer cell lines was observed). These highly cytotoxic activity may be back to the presence of the bi-apigenin derivatives; amentoflavone and Cupressuflavone that displayed unique binding docking score towards the active site of human DNA topoisomerase enzyme. The present study is a distinctive application for the biosynthesis of silver nanoparticles in a fast, simple, easy, eco-friendly, inexpensive and effective way.