Journal
DIABETOLOGIA
Volume 58, Issue 10, Pages 2254-2258Publisher
SPRINGER
DOI: 10.1007/s00125-015-3696-3
Keywords
Cholecystokinin; Enteroendocrine cells; Ghrelin; Glucagon-like peptide-1; Glucose-dependent insulinotropic polypeptide; Immunohistochemistry; Obesity; PeptideYY; Quantitative polymerase chain reaction; Roux-en-Y gastric bypass; Type 2 diabetes
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Funding
- Novo Nordisk Foundation
- Danish National Advanced Technology Foundation
- NNF Center for Basic Metabolic Research [Holst Group] Funding Source: researchfish
- Novo Nordisk Fonden [NNF12OC1015904, NNF11OC1014545] Funding Source: researchfish
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Aims/hypothesis We studied the impact of Roux-en-Y gastric bypass (RYGB) on the density and hormonal gene expression of small-intestinal enteroendocrine cells in obese patients with type 2 diabetes. Methods Twelve patients with diabetes and 11 age- and BMI-matched controls underwent RYGB followed by enteroscopy similar to 10 months later. Mucosal biopsies taken during surgery and enteroscopy were immunohistochemically stained for glucagon-like peptide-1 (GLP-1), peptide YY (PYY), cholecystokinin (CCK), glucose-dependent insulinotropic polypeptide (GIP) and prohormone convertase 2 (PC2) and the expression of GCG (encoding preproglucagon), PYY, CCK, GIP, GHRL (encoding ghrelin), SCT (encoding secretin), NTS (encoding neurotensin) and NR1H4 (encoding farnesoid X receptor) was evaluated. Results The density of cells immunoreactive for GLP-1, CCK and GIP increased in patients after RYGB and the density of those immunoreactive for GLP-1, PYY, CCK and PC2 increased in controls. In both groups, GHRL, SCT and GIP mRNA was reduced after RYGB while PYY, CCK, NTS and NR1H4 gene expression was unaltered. GCG mRNA was upregulated in both groups. Conclusions/interpretation Numerous alterations in the distribution of enteroendocrine cells and their expression of hormonal genes are seen after RYGB and include increased density of GLP-1-, PYY-, CCK-, GIP- and PC2-positive cells, reduced gene expression of GHRL, SCT and GIP and increased expression of GCG.
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