Journal
JOURNAL OF MOLECULAR MEDICINE-JMM
Volume 95, Issue 11, Pages 1227-1236Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00109-017-1585-6
Keywords
Crohn's disease; Bacterial translocation; Interleukin 26
Funding
- Asociacion Espanola de Gastroenterologia
- Swiss National Foundation [SNSF 310030_146290]
Ask authors/readers for more resources
Interleukin IL26 supports killing of microbes and the innate sensing of bacterial-derived DNA (bactDNA). We evaluated the relationship between IL26 serum levels and bactDNA translocation in Crohn's disease (CD). We ran a prospective study on CD patients in remission. IL26 common polymorphisms, serum cytokines and complement protein, amplified-bactDNA, and anti-TNF-alpha were evaluated. In vitro PBMC analysis was performed. Three hundred and thirteen patients were included (mean CDAI: 83.6 +/- 32.8; mean fecal calprotectin: 55.4 +/- 35.3 mu g/g). A total of 106 patients (33.8%) showed bactDNA and 223 patients (71%) had a varIL26 genotype. BactDNA significantly correlated with increased IL26 levels compared with bactDNA-negative patients. PBMCs from varIL26 patients significantly reduced E. coli killing capacity compared with wtIL26-genotyped patients. The stimulation with a recombinant IL26 protein reduced pro-inflammatory cytokines in response to E. coli in the varIL26 cell supernatants. Serum anti-TNF-alpha levels in varIL26 vs wtIL26-genotyped patients on biologics were significantly lower in the presence of bactDNA. Cells from varIL26 vs wtIL26-genotyped patients cultured with E. coli DNA and infliximab showed a significant decrease in free anti-TNF-alpha concentration. A varIL26 genotype was associated with the initiation of anti-TNF-alpha in CD patients during the 6-month follow-up. IL26 polymorphisms may prevent bactDNA clearance and identify CD patients with a worse inflammatory evolution and response to therapy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available