Journal
BIOENGINEERING-BASEL
Volume 6, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/bioengineering6040099
Keywords
alcohol dehydrogenase; enzyme immobilization; flow chemistry; process design
Funding
- European Union (EU) within the EU-Research Project ONEFLOW (Catalyst Cascade Reactions in 'One-Flow' within a Compartmentalized, Green-Solvent 'Digital Synthesis Machinery'-End-to-End Green Process Design for Pharmaceuticals) [737266]
Ask authors/readers for more resources
Flow processes and enzyme immobilization have gained much attention over the past few years in the field of biocatalytic process design. Downstream processes and enzyme stability can be immensely simplified and improved. In this work, we report the utilization of polymer network-entrapped enzymes and their applicability in flow processes. We focused on the superabsorber-based immobilization of an alcohol dehydrogenase (ADH) from Lactobacillus brevis and its application for a reduction of acetophenone. The applicability of this immobilization technique for a biotransformation running in a packed bed reactor was then demonstrated. Towards this end, the immobilized system was intensively studied, first in a batch mode, leading to >90% conversion within 24 h under optimized conditions. A subsequent transfer of this method into a flow process was conducted, resulting in very high initial conversions of up to 67% in such a continuously running process.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available