Journal
ENGINEERING REPORTS
Volume 1, Issue 5, Pages -Publisher
WILEY
DOI: 10.1002/eng2.12067
Keywords
boost; diurnal temperature shifts; microalgae; neutral lipids; nitrogen limitation; stress
Categories
Funding
- Carl Tryggers Stiftelse for Vetenskaplig Forskning [CTS 16:270]
- CementaHeidelberg AB
- Energimyndigheten [44677-1]
- Familjen Kamprads Stiftelse [20160169]
- Kalmar Energi
- Kalmarsundsregionens renhallare (KSRR)
- SMA Mineral
- Stiftelsen for Kunskaps-och Kompetensutveckling [20150219]
- Stiftelsen Lantbruksforskning [SLF O-15-20-559]
- Svenska Forskningsradet Formas [2018-00692]
- Formas [2018-00692] Funding Source: Formas
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Algal lipids have been observed to increase during autumn conditions (low light, low mean temperature, and diurnal shift in temperature), in large-scale outdoor photobioreactors. In this paper, we tested the effect of diurnal shifts in temperature (DS) and nitrogen (N) limitation on algal BODIPY lipid fluorescence cell(-1) (BPF). We show that DS increased BPF in algal biomass up to 28% more compared with N limitation, the standard stressor to boost neutral lipids (NL) in commercial production. Biomass yield was constant, regardless the DS range (6 degrees C-12 degrees C). A combination of both stressors had an additive effect on algal BPF. A polyculture from an outdoor photobioreactor was cultivated under controlled conditions at different regimes of light, temperature, and N limitation. DSs were mimicking autumn conditions with a difference of 6 degrees C, 10 degrees C, and 12 degrees C between day and night. Biomass and BPF were monitored over one to two weeks, and NLs were stained with a fluorescent marker (BODIPY) and detected with flow cytometry. Results indicate that, during autumn conditions, daily heating and cooling processes in contrast to N limitation do not challenge the trade-off between biomass production and BPF. During seasons when day temperature is still relatively high, DSs are rapid BPF boosting stressors, while N limitation could be applied to boost BPF further during other seasons.
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