Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 429, Issue 20, Pages 3031-3042Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2017.09.002
Keywords
tetranucleosomes; chromatin structure; nucleosomes
Categories
Funding
- iGE3
- European Research Council [322,778]
- Swiss National Science Foundation SNF Professorship [PP00P3_139137, PP00P3_163760]
- Republic and Canton of Geneva
- Fondation Ernst et Lucie Schmidheiny
- Fonds Constantin Topali
- Societe Academique de Geneve
- Swiss National Science Foundation (SNF) [PP00P3_163760, PP00P3_139137] Funding Source: Swiss National Science Foundation (SNF)
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Chromatin fiber organization is implicated in processes such as transcription, DNA repair and chromosome segregation, but how nucleosomes interact to form higher-order structure remains poorly understood. We solved two crystal structures of tetranucleosomes with approximately 11-bp DNA linker length at 5.8 and 6.7 angstrom resolution. Minimal intramolecular nucleosome-nucleosome interactions result in a fiber model resembling a flat ribbon that is compatible with a two-start helical architecture, and that exposes histone and DNA surfaces to the environment. The differences in the two structures combined with electron microscopy reveal heterogeneous structural states, and we used site-specific chemical crosslinking to assess the diversity of nucleosome-nucleosome interactions through identification of structure-sensitive crosslink sites that provide a means to characterize fibers in solution. The chromatin fiber architectures observed here provide a basis for understanding heterogeneous chromatin higher-order structures as they occur in a genomic context. (C) 2017 Elsevier Ltd. All rights reserved.
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