Journal
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
Volume 51, Issue 4, Pages 456-464Publisher
ELSEVIER TAIWAN
DOI: 10.1016/j.jmii.2017.01.005
Keywords
Japanese encephalitis; virus; NS5; Interferon; Anti-apoptosis; Hsp70
Categories
Funding
- Ministry of Science and Technology, Taiwan [MOST102-2320-B-039-044-MY3, MOST105-2320-B-039-053-MY3]
- China Medical University [CMU102-ASIA-15, CMU103-ASIA-07, CMU105-ASIA-10]
Ask authors/readers for more resources
Background: Japanese encephalitis virus (JEV) non-structural protein 5(NS5)exhibits type I interferon (IFN) antagonists, contributing to immune escape, and even inducing viral anti-apoptosis. This study investigated the anti-apoptotic mechanism of JEV NS5 protein on type I IFN-induced apoptosis of human medulloblastoma cells. Methods: Vector control and NS5-expressing cells were treated with IFN-beta, and then harvested for analyzing apoptotic pathways with flow cytometry, Western blotting, subcellular localization, etc. Results: Annexin V-FITC/PI staining indicated that IFN-beta triggered apoptosis of human medulloblastoma cells, but JEV NS5 protein significantly inhibited IFN-beta-induced apoptosis. Phage display technology and co-immunoprecipitation assay identified the anti-apoptotic protein Hsp70 as a NS5-interacting protein. In addition, Western blotting demonstrated that NS5 protein up-regulated the Hsp70 expression, and reduced IFN-beta-induced phosphorylation of ERK2, p38 MAPK and STAT1. Hsp70 down-regulation by quercetin significantly recovered IFN-beta-
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available