Journal
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume 27, Issue 1, Pages 92-100Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1608.08063
Keywords
meso-2,3-butanediol dehydrogenase; meso-2,3-butanediol; (3R)-acetoin; NAD(+) regeneration; vitreoscilla hemoglobin; whole-cell biocatalysis
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Funding
- National Natural Science Foundation of China [31301858, 81673542]
- New Century Excellent Talents Supporting Plan of the Provincial Education Department of Fujian Province of China [K8015056A]
- Outstanding Youth Plan of the Provincial Education Department of Fujian Province of China [JA14100]
- Outstanding Youth Plan of Fujian Agriculture and Forestry University [xjq201408]
- KU Research Professor Program of Konkuk University
- KU Brain Pool Fellowship of Konkuk University
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Acetoin (AC) is a volatile platform compound with various potential industrial applications. AC contains two stereoisomeric forms: (3S)-AC and (3R)-AC. Optically pure AC is an important potential intermediate and widely used as a precursor to synthesize novel optically active materials. In this study, chiral (3R)-AC production from meso-2,3-butanediol (meso-2,3BD) was obtained using recombinant Escherichia coli cells co-expressing meso-2,3-butanediol dehydrogenase (meso-2,3-BDH), NADH oxidase (NOX), and hemoglobin protein (VHB) from Serratia sp. T241, Lactobacillus brevis, and Vitreoscilla, respectively. The new biocatalyst of E. coli/pET-mbdh-nox-vgb was developed and the bioconversion conditions were optimized. Under the optimal conditions, 86.74 g/l of (3R)-AC with the productivity of 3.61 g/l/h and the stereoisomeric purity of 97.89% was achieved from 93.73 g/l meso-2,3-BD using the whole-cell biocatalyst. The yield and productivity were new records for (3R)-AC production. The results exhibit the industrial potential for (3R)-AC production via whole-cell biocatalysis.
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