Journal
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume 27, Issue 11, Pages 2070-2073Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1707.07073
Keywords
SARS; helicase; ATP hydrolysis; dsDNA unwinding; cytotoxicity
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Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2017R1A2B4003251]
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We have discovered a novel chemical compound, (E)-3-(furan-2-yl)-N-(4-sulfamoylphenyl) acrylamide, that suppresses the enzymatic activities of SARS coronavirus helicase. To determine the inhibitory effect, ATP hydrolysis and double-stranded DNA unwinding assays were performed in the presence of various concentrations of the compound. Through these assays, we obtained IC50 values of 2.09 +/- 0.30 mu M (ATP hydrolysis) and 13.2 +/- 0.9 mu M (DNA unwinding), respectively. Moreover, we found that the compound did not have any significant cytotoxicity when 40 mu M of it was used. Our results showed that the compound might be useful to be developed as an inhibitor against SARS coronavirus.
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