Journal
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume 27, Issue 7, Pages 1276-1280Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1611.11021
Keywords
mcr-1; knockout; E. coli; CRISPR/Cas9 system; BMAP-27
Categories
Funding
- National Natural Science Foundation of China [31302009]
- Natural Science Foundation of Jiangsu Province [BK20160585]
- Jiangsu Agricultural Science and Technology Innovation Fund [CX(16)1060]
- Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base Self-determined Project [3201614]
- Zhejiang Province Major Program [2015C02041]
- National Agricultural Product Quality and Safety Risk Assessment [GJGP201701203]
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The mcr-1 gene is a new superbug gene discoverd in China in 2016 that makes bacteria highly resistant to the last-resort class of antibiotics. The mcr-1 gene raised serious concern about its possible global dissemination and spread. Here, we report a potential anti-resistant strategy using the CRISPR/Cas9-mediated approach that can efficiently induce mcr-1 gene knockout in Escherichia coli. Our findings suggested that using the CRISPR/Cas9 system to knock out the resistance gene mcr-1 might be a potential anti-resistant strategy. Bovine myeloid antimicrobial peptide-27 could help deliver plasmid pCas:: mcr targeting specific DNA sequences of the mcr-1 gene into microbial populations.
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