4.4 Article

Proteome Changes in Penicillium expansum Grown in a Medium Derived from Host Plant

Journal

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume 27, Issue 3, Pages 624-632

Publisher

KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1605.05022

Keywords

Penicillium expansum; apple juice medium; proteomics; fungi-host interaction

Funding

  1. National Key Technology Research and Development Program of China [2015BAK45B00]
  2. Open Fund of Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture [SWDSJC2013001]
  3. Practice Innovation Training Program Project of College Students in Jiangsu Province, China [201510299011Z]

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Penicillium expansum causes blue mold rot, a prevalent postharvest disease of pome fruit, and is also the main producer of the patulin. However, knowledge on the molecular mechanisms involved in this pathogen-host interaction remains largely unknown. In this work, a twodimensional gel electrophoresis-based proteomic approach was applied to probe changes in P. expansum 3.3703 cultivated in apple juice medium, which was used to mimic the in planta condition. The results showed that the pH value and reducing sugar content in the apple juice medium decreased whereas the patulin content increased with the growing of P. expansum. A total of 28 protein spots that were up-regulated in P. expansum when grown in apple juice medium were identified. Functional categorization revealed that the identified proteins were mainly related to carbohydrate metabolism, secondary metabolism, protein biosynthesis or degradation, and redox homeostasis. Remarkably, several induced proteins, including glucose dehydrogenase, galactose oxidase, and FAD-binding monooxygenase, which might be responsible for the observed medium acidification and patulin production, were also detected. Overall, the experimental results provide a comprehensive interpretation of the physiological and proteomic responses of P. expansum to the host plant environment, and future functional characterization of the identified proteins will deepen our understanding of fungi-host interactions.

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