Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 133, Issue -, Pages 69-75Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2016.12.005
Keywords
Pleurocidin antimicrobial peptide; Microbial detection; Piezoelectric sensor
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Funding
- National Natural Science Foundation of China [21275042]
- National High Technology Research and Development Program of China (863 Program) [2013AA020203]
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The rapid detection of microbes is critical in clinical diagnosis and food safety. Culture-dependent assays are the most widely used microbial detection methods, but these assays are time-consuming. In this study, a rapid microbial detection method was proposed using a pleurocidin/single-walled carbon nanotubes/interdigital electrode-multichannel series piezoelectric quartz crystal (pleurocidin/SWCNT/IDE-MSPQC) sensor. The selected pleurocidin antimicrobial peptide served as a recognition probe that exhibits broad-spectrum antimicrobial activity and the SWCNT acted as the electronic transducer and cross-linker for the immobilization of pleurocidin on the IDE. The response mechanism of the sensor was based on the specific interaction between pleurocidin and the microbe causing pleurocidin to detach from the SWCNT modified IDE, resulting in a sensitive frequency shift response of the MSPQC. Microbes that may be clinically present in the bloodstream during an infection were successfully detected by the proposed method within 15 min. The developed strategy provides a new universal platform for the rapid detection of microbes. (C) 2016 Elsevier B.V. All rights reserved.
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