4.3 Article

Comparison of the XTT and resazurin assays for quantification of the metabolic activity of Staphylococcus aureus biofilm

Journal

JOURNAL OF MICROBIOLOGICAL METHODS
Volume 139, Issue -, Pages 135-137

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2017.06.004

Keywords

S. aureus; Biofilm; Resazurin; XTT; Metabolic activity

Funding

  1. Miguel Servet Program (ISCIIIMICINN) of the Health Research Fund (FIS) of the Carlos III Health Institute (ISCIII), Madrid, Spain [CP13/00268]
  2. Consejeria de Educacion, Juventud y Deporte de la Comunidad de Madrid and Fondo Social Europeo [PEJ15/BIO/AI-0406]
  3. ISCIII [PI14/01933]
  4. European Regional Development Fund (FEDER)

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We assessed whether resazurin was as efficient as XTT in the measurement of the metabolic activity of 209 clinical Staphylococcus aureus biofilm using an vitro model comparing the percentage of formazan and resorufin. The overall categorical agreement was 61.2% (r = 0.024), which means that resazurin can not substitute XTT.

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