☆ 4.3 Article

Live-cell imaging of Salmonella Typhimurium interaction with zebrafish larvae after injection and immersion delivery methods

JOURNAL OF MICROBIOLOGICAL METHODS (2017)

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Journal

JOURNAL OF MICROBIOLOGICAL METHODS
Volume 135, Issue -, Pages 20-25

Publisher

ELSEVIER
DOI: 10.1016/j.mimet.2017.01.020

Keywords

Host-pathogen interaction; Danio rerio; Zebrafish; Salmonella; Live-cell imaging; Infection; Immune response

Categories

Biochemical Research Methods Microbiology

Funding

  1. FONDECYT [1120209, 1140754]
  2. CONICYT [21120431, 21130717]
  3. FONDAP [15090007]

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The zebrafish model has been used to determine the role of vertebrate innate immunity during bacterial infections. Here, we compare the in vivo immune response induced by GFP-tagged Salmonella Typhimurium inoculated by immersion and microinjection in transgenic zebrafish larvae. Our novel infection protocols in zebrafish allow live-cell imaging of Salmonella colonization. (C) 2017 Elsevier B.V. All rights reserved.

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