Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 142, Issue -, Pages 79-82Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2017.09.011
Keywords
Dinoroseobacter shibae; Bacillus subtilis; Sybr Green I; DiOC(2)(3); Propidium iodide; Fluorescence microscopy
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Live-dead staining with propidium iodide can give erroneous results for bacteria showing high membrane potentials. We observed uptake of propidium ions across intact cell membranes for Dinoroseobacter shibae and Bacillus subtilis. Apparently, a high membrane potential facilitates breakthrough of the double-charged propidium ion and can mark viable cells as dead.
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