Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 142, Issue -, Pages 46-51Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2017.08.020
Keywords
Biofilms; S. aureus; Periprosthetic joint infection; Orthopaedic surgery; Hospital-acquired infections
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Funding
- British Hip Society, London, United Kingdom
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In vitro biofilm assays are a vital first step in the assessment of therapeutic effectiveness. Current biofilm models have been found to be limited by throughput, reproducibility, and cost. We present a novel in vitro biofilm model, utilising a sodium alginate substratum for surface biofilm colony formation, which can be readily dissolved for accurate evaluation of viable organisms. The dissolving bead biofilm assay was evaluated using a range of clinically relevant strains. The reproducibility and responsiveness of the assay to an antimicrobial challenge was assessed using standardised methods. Cryo-scanning electron microscopy was used to image biofilm colonies. Biofilms were grown for 20 h prior to testing. The model provides a reproducible and responsive assay to clinically-relevant antimicrobial challenges, as defined by established guidelines. Moreover cryo-scanning electron microscopy demonstrates that biofilm formation is localised exclusively to the alginate bead surface. Our results suggest that this simple model provides a robust and adaptable assay for the investigation of bacterial biofilms.
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