Journal
JOURNAL OF MEDICINAL CHEMISTRY
Volume 61, Issue 2, Pages 599-618Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jmedchem.7b00675
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Funding
- European Research Council [ERC-2012-StG-311460 DrugE3CRLs]
- UK Biotechnology and Biological Sciences Research Council BBSRC [BB/G023123/2]
- European Commission [PIEF-GA-2012-328030]
- Wellcome Trust [102398/Z/13/Z, 100476/Z/12/Z, 094090/Z/10/Z, 097945/B/11/Z]
- Fundacao para a Ciencia e Tecnologia FCT [SFRH/BD/101598/2014]
- Cancer Research UK [C99667/A12918]
- Wellcome Trust [102398/Z/13/Z] Funding Source: Wellcome Trust
- BBSRC [BB/G023123/2] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/G023123/2] Funding Source: researchfish
- Cancer Research UK [12918] Funding Source: researchfish
- Fundação para a Ciência e a Tecnologia [SFRH/BD/101598/2014] Funding Source: FCT
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The von Hippel-Lindau tumor suppressor protein is the substrate binding subunit of the VHL E3 ubiquitin ligase, which targets hydroxylated alpha subunit of hypoxia inducible factors (HIFs) for ubiquitination and subsequent proteasomal degradation. VHL is a potential target for treating anemia and ischemic diseases, motivating the development of inhibitors of the VHL:HIF-alpha protein-protein interaction. Additionally, bifunctional proteolysis targeting chimeras (PROTACs) containing a VHL ligand can hijack the E3 ligase activity to induce degradation of target proteins. We report the structure-guided design and group-based optimization of a series of VHL inhibitors with low nanomolar potencies and improved cellular permeability. Structure-activity relationships led to the discovery of potent inhibitors 10 and chemical probe VH298, with dissociation constants <100 nM, which induced marked HIF-1 alpha intracellular stabilization. Our study provides new chemical tools to probe the VHL-HIF pathways and new VHL ligands for next-generation PROTACs.
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