Journal
ANALYTICAL METHODS
Volume 11, Issue 44, Pages 5661-5672Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9ay01988j
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Funding
- Tabriz University of Medical Sciences, Tabriz-Iran
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A novel sandwich-type biosensor based on a signal amplification strategy of various nanocomposites was developed to monitor the prostate-specific antigen (PSA) in real samples. The engineered immunosensor was fabricated using iron oxide magnetic nanoparticles modified with a polymer poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) [Fe3O4/PEDOT:PSS] conductive nanocomposite that utilized the immobilization of primary antibodies (biotinylated antibody (Ab1)). In order to improve the electron transfer rate, a cysteamine-capped gold nanoparticle (AuNPs-CysA) was applied for conjugation with a secondary antibody (HRP-Ab2). The fabrication procedure was investigated by square wave voltammetry. Under optimized experimental conditions, the calibration curve showed a wide linear range (0.001-0.5 mu g L-1) for the PSA biomarker with a low limit of quantification of 0.001 mu g L-1. Finally, satisfactory results were obtained for the determination of PSA in untreated human plasma samples, indicating the potential of the immunoassay for applications in clinical bioanalysis.
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