Journal
CHEMICAL SCIENCE
Volume 10, Issue 45, Pages 10607-10619Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9sc03450a
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Funding
- Wellcome Trust [092506, 098335]
- MRC [MR/M008991/1]
- EPSRC [G0025101, EP/M020983/1]
- EPSRC [EP/M020983/1] Funding Source: UKRI
- MRC [MR/M008991/1] Funding Source: UKRI
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Monosaccharides, such as glucose and fructose, are important to life. In this work we highlight how the rapid delivery of improved C-13 detectability for sugars by nuclear magnetic resonance (NMR) can be achieved using the para-hydrogen based NMR hyperpolarization method SABRE-Relay (Signal Amplification by Reversible Exchange-Relay). The significant C-13 signal enhancements of 250 at a high field of 9.4 T, and 3100 at a low field of 1 T, enable the detection of trace amounts of these materials as well as the quantification of their tautomeric makeup. Using studies on C-13 and H-2 isotopically labelled agents we demonstrate how hyperpolarization lifetime (T-1) values can be extended, and how singlet states with long lifetimes can be created. The precise quantification of d-glucose-C-13(6)-d(7) at the millimolar concentration level is shown to be possible within minutes in conjunction with a linear hyperpolarized response as a function of concentration. In addition to the measurements using labelled materials, low concentration detection is also illustrated for millimolar samples with natural abundance C-13 where isomeric form quantification can be achieved with a single transient.
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